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Test for ketonuria ( Rothera's test )

Test for ketonuria ( Rothera's test )

Apparatus

Test tube, Urine, Solid ammonium sulfate salt, Sodium nitroprusside, liquor ammonia.

Principle

Ketone bodies combine with alkaline solution of sodium nitroprusside formed purple complex.

Procedure

  • Take 5ml urine in a test tube.
  • Saturate it with solid ammonium salt, it will start settling to the bottom of the tube when it saturated.
  • Add a few crystals of sodium nitroprusside and shake.
  • Add liquor ammonia in test tube with the side of the inner wall.
Rothera's test, test for ketonuria, Aas, alfaaz-e-aas
Rothera's test

Results

Appearance of the purple coloured ring at the junction indicates presence of ketone bodies.

Causes of ketonuria

  • Diabetes ketoacidosis
  • Dehydration
  • Hyperemesis gravidarum
  • Fever
  • Cachexia
  • After general anaesthesia
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