Test for Bilirubin / Bile pigment in urine ( Fouchet's test for bilirubinuria )

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Test for bilirubin / bile pigment in urine ( Fouchet's test )

Bilirubin is breakdown product of haemoglobin.
Normally no bilirubin passed in urine.

Apparatus

Test tube, 10% barium chloride, filter paper, dropper, fouchet's reagent, urine.

Principal

Oxidises bilirubin convert into green biliverdin with the help of ferric chloride.

Procedure

  • Take 10ml of urine in a test tube.
  • Add 3 -5 ml 10% barium chloride.
  • Filter through filter paper.
  • To precipitate on filter paper, add a few drops of fouchet's reagent. 
Fouchet's reagent = Ferric chloriode + Trichloroacetic acid

Result

Development of green colour indicates bilirubin.

Causes of bilirubinuria

  1. Obstructive jaundice
  2. Hepatocellular jaundice. 
Location:India India

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