Skip to main content

Test for Bilirubin / Bile pigment in urine ( Fouchet's test for bilirubinuria )

Test for bilirubin / bile pigment in urine ( Fouchet's test )

Bilirubin is breakdown product of haemoglobin.
Normally no bilirubin passed in urine.

Apparatus

Test tube, 10% barium chloride, filter paper, dropper, fouchet's reagent, urine.

Principal

Oxidises bilirubin convert into green biliverdin with the help of ferric chloride.

Procedure

  • Take 10ml of urine in a test tube.
  • Add 3 -5 ml 10% barium chloride.
  • Filter through filter paper.
  • To precipitate on filter paper, add a few drops of fouchet's reagent. 
Fouchet's reagent = Ferric chloriode + Trichloroacetic acid

Result

Development of green colour indicates bilirubin.

Causes of bilirubinuria

  1. Obstructive jaundice
  2. Hepatocellular jaundice. 
Labels:

Comments

Post a Comment

Please do not enter any spam link in the comment box

Popular posts from this blog

Histology text & Atlas by Brijesh kumar

Histology text & Atlas by Brijesh kumar Download book pdf About file:- File type - pdf File size - 26.35 MB Pages - 392 Language - English 
Post a Comment
Read more

Lysosomes

Name :- Lysosomes, garbage system of the cell, suicidal bag of the cell Properties : These are membrane bound vesicular structures founds throughout the cytoplasm. These are formed by the process of packaging in the golgi apparatus. Enzymes of lysosomes are synthesized in rough endoplasmic reticulum. Among the organelles, the lysosomes have the thickest covering membrane that is formed by a bilayered lipid material . Lysosomes have 50 different hydrolytic enzymes, known as acid hydroxylases. Types of Lysosomes : Primary lysosomes, this is an inactive of form of lysosomes. Secondary lysosomes, this is an active form of lysosomes (lysosomes active during phagosome or endosome and at this time lysosomes pH become acidic and enzymes are activate). Lysosomal Enzymes :   Proteases :- which hydrolyze the proteins into amino acids. Lipases :- which hydrolyze the lipids into fatty acids and glycerides. Amylases :- which hydrolyze the polysaccharides into glucose. Nucleases :- whi
Post a Comment
Read more

Practical Medicine (P. J. Mehta's)

Practical Medicine (P. J. Mehta's) 20th edition CONTENTS PART-1 CLINICAL CASES 1. History Taking and Symptomatology 2. General Examination 3. Abdomen 4. Respiratory System 5. Cardiovascular System 6. Central Nervous System PART-2 TABLE-WORK 7. Medical Emergencies 8. Electrocardiography 9. Radiology 10. Instruments 11. Procedures 12. Hematology 13. Clinical Pathology 14. Pathology Specimens 15. Drugs ABOUT BOOK Name Book Practical Medicine (P. J. Mehta’s) Author/ Editior (20 th edition) SP Mehta, SR Joshi, Nihar P Mehta / Dr. Hardik shah, Dr. Sunita Iyer, Dr. Nikesh Jain, Dr. Rajiv Shah. Type pdf. Size 69.47 MB Pages 630 Pages type Colored Quality (Good/average/bad) Good D ownload Now Similar Posts :- 1.  Park’s Textbook of PSM 2.  Review of Preventive and Social Medicine (Vivek Jain) 3.  Medicine (Exam Preparatory
Post a Comment
Read more